![]() ![]() Western Blotting Analysis: A 1:16,000 dilution of this antibody detected GAPDH in HEK293 cell lysate. Uncharacterized bands may be observed in some lysate(s).Įvaluated by Western Blotting in HEK293 cell lysate. ![]() Target molecular weight ~38 kDa observed 36.05 kDa calculated. SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Note=Postnuclear and Perinuclear regions. Many pseudogenes similar to this locus are present in the human genome.įUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway. The enzyme exists as a tetramer of identical chains. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Predicted to react with Canine, Horse based on 100% sequence homology. It targets an epitope within 12 amino acids from the internal region. This chicken polyclonal antibody specifically detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Please refer to the Certificate of Analysis for the lot-specific concentration. KLH-conjugated linear peptide corresponding to 12 amino acids from the internal region of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user Western Blotting Analysis: A 1:16,000 dilution from a representative lot detected GAPDH in NIH3T3 cell lysate. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western Blotting. Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.Īnti-GAPDH, Cat. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. In the nucleus, it participates in transcription, RNA transport, DNA replication, and apoptosis. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1 that contains a nuclear localization signal. It also plays a role in several nuclear functions. It is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. GAPDH is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities and is considered as a 'house-keeping' enzyme. Glyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406 also known as EC: 1.2.1.12, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. glyceraldehyde-3-phosphate dehydrogenase.glyceraldehyde 3-phosphate dehydrogenase.100% Performance Guaranteed Key Spec Table Species Reactivity ![]()
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